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I. Specialized equipment for cell-culturing of
different cell-lines
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Equipment
includes all instruments ranging from autoclaves
for sterilization of glass-ware to safety hoods
and incubators for manipulation and growth of
cells, and low temperature freezers for storage.
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Our bio-safety cabinet, from
Nuaire, of class 10 quality with UV sterilization
ability. The bio-safety cabinet provides the high
sterile environment needed for working with environmentally
sensitive biological samples and live cells. Manipulation
of cells outside of incubators or protected environments
is done in this hood to ensure isolation between
the sample and the experimentalist.
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The
autoclave we use for sterilization of cell-culture
lab ware and fluids utilizing super heated steam
(~ 105°C to 135°C) at high temperature
and high pressure. This piece of equipment is
imperative for any cell culture work. Our autoclave
(from Hirayama) with a capacity of up to 50 liters
can sterilize solid substances such as glass,
ceramics, metal or rubber etc. and also liquids
such as water, reagents and various mediums etc.
It is also used to liquefy different media.
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Our
range of incubators, which includes one bench
top shaking incubator from VWR Scientific and
one incubated and refrigerated floor shaker from
Barnstead International. Temperature control in
the range of ~ ambient - 15°C to 70°C
is available. This provides physiological and
sterile environments needed for cell growth in
culture.
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Our
-80°C freezer that is especially useful
for long term storage of cells and cell
stock solutions, DNA and protein storage
and also for various biological reagents
that require such low temperatures to remain
intact over long durations.
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